On the basis of the design of the ordinary PCR instrument, a fluorescence signal excitation and acquisition system and a computer analysis and processing system are added to form an instrument with fluorescent quantitative PCR function. The principle of PCR amplification is the same as that of conventional PCR amplification. The primers added in PCR amplification are labeled by isotope and fluorescein. The primers and fluorescent probes are used to bind and amplify the template. The amplification results are collected by the real-time signal collection system, and the signals are connected and transmitted to the computer analysis and processing system, and the quantitative real-time output is obtained.Back to list
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