Reagent salesperson | ||
---|---|---|
Tel: | 0571-87774513 | |
E-mail: | reagent@bioer.com.cn |
Aquatic Pathogen Series
In this series of products, primers and TaqMan probes are designed with virus specific gene fragments to realize rapid qualitative detection of viral nucleic acids in aquatic and economic animals such as shrimp and fish. It can be used for virus tracking detection in the breeding process, and also for environmental monitoring, so as to avoid the spread of virus and reduce economic losses. The series also has multi-channel reagents that simultaneously detect two diseases in the same tube.
Main component |
Storage temperature |
|
Amplification reagents |
PCR reaction reagents |
-15~-25℃ |
Primers probe |
||
Control |
Negative control |
|
Positive control |
Product list
Cat# |
Product Name |
Package |
Note |
BSN01S1 |
White Spot Syndrome Virus Real Time PCR Detection Kit |
16T |
-20℃Storage |
BSN01M1 |
32T |
||
BSN02S1 |
Infectious hypodermal and haematopoietic necrosis virus Real Time PCR Detection Kit |
16T |
|
BSN02M1 |
32T |
||
BSN03S1 |
Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit |
16T |
|
BSN03M1 |
32T |
||
BSN04S1 |
Vibrio Harveyi Real Time PCR Detection Kit |
16T |
|
BSN04M1 |
32T |
||
BSN05S1 |
EMS & EHP Dual Chanel Real Time PCR Detection kit |
16T |
|
BSN05M1 |
32T |
||
BSN06S1 |
Bacteria & Vibrio Dual Chanel Real Time PCR Detection Kit |
16T |
|
BSN06M1 |
32T |
||
BSN07S1 |
Taura Syndrome Virus Real Time PCR Detection Kit |
16T |
|
BSN07M1 |
32T |
||
BSN08S1 |
Yellow head virus Real Time PCR Detection Kit |
16T |
|
BSN08M1 |
32T |
||
BSN09S1 |
Enterocytozoon hepatopenaei Real Time PCR Detection Kit |
16T |
|
BSN09M1 |
32T |
||
BSN10S1 |
Acute Hepatopancreatic Necrosis Disease Real Time PCR Detection Kit |
16T |
|
BSN10M1 |
32T |
1. It is suitable for the Bioer fluorescence quantitative PCR detection system and the same kind of real-time quantitative detection system
2. High sensitivity: detection limit up to 100 copies/mL
3. High specificity: The design of specific primers and probes can safely avoid cross reactions and the resulting false positives
4. Easy to operate, short time consuming: the reagent adopts premixed form, less components, easy to operate, only need about 1 hour to complete whole experiment.
5. DUTP-UNG system is used to effectively prevent contamination of PCR products
Experiment 1
White Spot Syndrome Virus Real Time PCR Detection Kit
(1) Linear tests with concentrations of 102 to 107 copies/mL of DNA showed good results, R2=0.9985
DNA(copies/mL) |
Ct |
Ct value |
106 |
19.18 |
19.2 |
19.22 |
||
105 |
22.64 |
22.63 |
22.62 |
||
104 |
26.31 |
26.37 |
26.43 |
||
103 |
29.82 |
29.83 |
29.84 |
||
102 |
34.1 |
34.11 |
34.12 |
|
(2) The test was repeated using lower concentrations of DNA
The results showed that the detection rate of low concentration DNA was 100%, and the reproducibility was good
Experiment 2
Dual nucleic acid detection reagent linear test of acute hepatopancreas necrosis (EMS) and hepatoenterocytic worm (EHP) (DNA concentration 102 ~ 107copies/mL)
DNA(copies/mL) |
EHP(FAM) |
EMS(HEX) |
106 |
16.93 |
16.42 |
105 |
20.3 |
19.95 |
104 |
24.12 |
23.41 |
103 |
27.73 |
27.22 |
102 |
31.62 |
31.48 |
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