Aquatic Pathogen Series

• Aquatic Pathogen Series Reagents MORE
  Product Details：

  In this series of products, primers and TaqMan probes are designed with virus specific gene fragments to realize rapid qualitative detection of viral nucleic acids in aquatic and economic animals such as shrimp and fish. It can be used for virus tracking detection in the breeding process, and also for environmental monitoring, so as to avoid the spread of virus and reduce economic losses. The series also has multi-channel reagents that simultaneously detect two diseases in the same tube.

   

  	Main component	Storage temperature
  Amplification reagents	PCR reaction reagents	-15～-25℃
  	Primers probe
  Control	Negative control
  	Positive control

   

  Product list
  

  Cat#	Product Name	Package	Note
  BSN01S1	White Spot Syndrome Virus Real Time PCR Detection Kit	16T	-20℃Storage
  BSN01M1		32T
  BSN02S1	Infectious hypodermal and haematopoietic necrosis virus Real Time PCR Detection Kit	16T
  BSN02M1		32T
  BSN03S1	Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit	16T
  BSN03M1		32T
  BSN04S1	Vibrio Harveyi Real Time PCR Detection Kit	16T
  BSN04M1		32T
  BSN05S1	EMS & EHP Dual Chanel Real Time PCR Detection kit	16T
  BSN05M1		32T
  BSN06S1	Bacteria & Vibrio Dual Chanel Real Time PCR Detection Kit	16T
  BSN06M1		32T
  BSN07S1	Taura Syndrome Virus Real Time PCR Detection Kit	16T
  BSN07M1		32T
  BSN08S1	Yellow head virus Real Time PCR Detection Kit	16T
  BSN08M1		32T
  BSN09S1	Enterocytozoon hepatopenaei Real Time PCR Detection Kit	16T
  BSN09M1		32T
  BSN10S1	Acute Hepatopancreatic Necrosis Disease Real Time PCR Detection Kit	16T
  BSN10M1		32T

   

  
  

  Product Features：

  1. It is suitable for the Bioer fluorescence quantitative PCR detection system and the same kind of real-time quantitative detection system

  2. High sensitivity: detection limit up to 100 copies/mL

  3. High specificity: The design of specific primers and probes can safely avoid cross reactions and the resulting false positives

  4. Easy to operate, short time consuming: the reagent adopts premixed form, less components, easy to operate, only need about 1 hour to complete whole experiment.

  5. DUTP-UNG system is used to effectively prevent contamination of PCR products

  Experimental data:

  Experiment 1

  White Spot Syndrome Virus Real Time PCR Detection Kit

  (1) Linear tests with concentrations of 102 to 107 copies/mL of DNA showed good results, R2=0.9985

  

  DNA（copies/mL）	Ct	Ct value
  106	19.18	19.2
  	19.22
  105	22.64	22.63
  	22.62
  104	26.31	26.37
  	26.43
  103	29.82	29.83
  	29.84
  102	34.1	34.11
  	34.12

   

   

   

  (2) The test was repeated using lower concentrations of DNA

  

   

  The results showed that the detection rate of low concentration DNA was 100%, and the reproducibility was good

  
  Experiment 2

   Dual nucleic acid detection reagent linear test of acute hepatopancreas necrosis (EMS) and hepatoenterocytic worm (EHP) (DNA concentration 10² ~ 10⁷copies/mL)

  

  DNA（copies/mL）	EHP（FAM）	EMS（HEX）
  106	16.93	16.42
  105	20.3	19.95
  104	24.12	23.41
  103	27.73	27.22
  102	31.62	31.48

   

  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  

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