Fast Lysis Method

• BioFast BIOZOL Total RNA Extraction Reagent MORE
  Product Details：

  Kit Components

  Cat#	BSC59M1
  Components	100Tests
  BIOZOL Total RNA Extraction Reagent	100 ml
  Handbook	1 copy
  RNA Grind tube	100 tubes

  Introduction

      BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

      Since the operation of BioFast Biozol is very easy, it can realize the extraction  for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

  Product Features：

  Storage and transportation

  •  1. Recommended to store at 2-8 ℃ and the quality guarantee period is up to one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the performance of the reagent.
  • 2. The kit can be transported at room temperature.

  Important Notes

      RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

   

  • ◆Always wear gloves and respirator in order to prevent RNA degradation.
  • ◆Use sterile, disposable plastic ware and automatic pipettes.
  • ◆All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6－8 hours, while tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temperature in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.
  • ◆Build a separate RNA laboratory with the only instruments if possible.

   

  Experimental data:

  • Absorbance analysis of yield and purity

  lPrepare RNA, dilute by 25mM Tris-HCl（pH7.5）, RNase-free water or TE buffer in a right factor；

  lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer；

  lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

  Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

  • The conversion factor for RNA is 0.040μg/μl per OD260 unit
  • Notice: the range of Spec reading A260： 0.1 260<1.0

  lCalculate the purity of RNA as follows: Ratio=(Spec.readingA260)/( Spec.readingA280)

  • Ration of 1.8~2.1 is considered ideal purity.

  （The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.）

  Pictures of Experiment

  Picture 1：Total RNA extracted from animal tissues

  Picture 2 Total RNA extracted from rice leaves

                      

  Manual download
• BioFast Simply P Total RNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC60S1
  Components	50Tests
  RNA Grind tube	50 tubes
  Solution R2	30ml
  Wash Buffer	18ml (add 54ml ethanol before use)
  Elution Buffer	10ml
  Spin columns	50
  Handbook	1copy

  Introduction

      Biofast-simplyP is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. Add sample to the grind tube with Solution R2 for homogenization and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.

      The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-through put. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+  selection、in  vitro  translation、RNase  protect  assay,  RT-PCR/Real  time  RT-PCR analysis、construction cDNA library etc.

  Product Features：

  Storage and transportation

  •  1. The kit has demonstrated stability of 24 months when stored at room temperature.
  •  2. The kit can be transported at room temperature.

  Apparatus and materials to be prepared by the user

  * Sterile 1.5ml microcentrifuge tubes             * 10µl/200µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm           * Absolute ethanol        *  Vortex mixer

  Important note

  Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

  Experimental data:

  Analysis RNA

  • Absorbance analysis of yield and purity

  lPrepared RNA will be dilute by 25mM Tris-HCl （pH7.5）, RNase-free water or TE buffer in a right factor；

  lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl（pH7.5）, RNase-free water or TE buffer；

  lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

  Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)

  1. The conversion factor for RNA is 0.040μg/μl per OD260 unit

  2. Notice: the range of Spec reading A260： 0.1≤OD260≤1.0

  Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/( Spec.readingA280)

  3. Ration of 1.8~2.0 are considered ideal purity.

  （The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.）

  Picture 1：Animal tissues                                     

             

  Picture 2：Rice leaf

           

  
  

  Manual download
• BioFast Soil Genomic DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC21S1
  Components	50Tests
  SP Buffer	45ml
  Lysis S Buffer	5ml
  DA Buffer	12.5ml
  Binding Buffer	35ml
  Wash Buffer	30ml(add 45ml absolute ethanol before use)
  Elution Buffer	15ml
  Grind Tube	50tubes
  Spin Column	50tubes
  Handbook	1copy

  Introduction

      The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

      At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

      Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

  Product Features：

  Storage

  All reagents, when stored properly, are stable for 18 months.

  Apparatus and Materials to Be Supplied by the User

  *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g              * Absolute ethanol

  Important notes

  • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
  • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

  Experimental data:

  Analysis DNA

  • Absorbance analysis

  Get some DNA，diluted in a advisable factor with elution buffer. Survey the OD260，OD280 and OD320.

  Expressions：concentration（μg/ml）＝50×OD260×dilution fact

  Target： 2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis

  0.8～1％ Agarose gel

  Real-time Taqman PCR detect

  
  

  Manual download
• BioFastSpin Plant Genomic DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC18S1
  Components	50Tests
  LP Buffer	45 ml
  DA Buffer	10 ml
  P Binding Buffer	16ml
  G Binding Buffer	25ml
  Wash Buffer	25ml
  Elution Buffer	10ml
  Spin Column	50
  Grind Tube	50
  Handbook	1 copy

  Introduction

      The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from plant tissues, Designed for use with the lysis instrument from BIOER，plants are easily lysed within 40 s.The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this Kit.

      The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

  Product Features：

  Storage

  • 1. The kit should be stored at 15-25℃.
  • 2.  All reagents, when stored properly, are stable for 18 months.

  Apparatus and Materials to Be Supplied by the User

  *  Sterile 1.5ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g              * Absolute ethanol            * Vortex mixer

  Important notes

  • 1. Please add 37.5ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
  • 2. Please add 32ml absolute ethanol to P Binding Buffer and mix thoroughly before the first use.
  • 3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

  Experimental data:

  Analysis DNA

  • Absorbance analysis

  Get some DNA，diluted in a advisable factor with Elution Buffer. Survey the OD260，OD280 and OD320.

  Expressions：concentration（μg/ml）＝50×OD260×dilution fact

  Target：   2.0≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis

  0.8～1％ Agarose gel

  Example 1：Rice leaves

  

  Example 2：Rice leaves

  

  
  

  Manual download
• BioFastSpin Tissue Genomic DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC20S1
  Components	50Tests
  FL Buffer	30ml
  Binding Buffer	35ml
  PW Buffer	10.5ml
  Wash Buffer	25.2ml
  Elution Buffer	10ml
  PK Solution	0.5ml
  WS buffer	5ml
  Grind Tube	50 tubes
  Spin Column	50 tubes
  Handbook	1 copy

  Introduction

      The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than1.5 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 30 μg genomic DNA can be acquired from up to 50 mg tissue by using this kit.

      The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

  Product Features：

  Storage

  • 1. The Protease K is to be stored at 2-8℃, others at 15-25℃.
  • 2. All reagents, when stored properly, are stable for 18 months.

  Apparatus and Materials to Be Supplied by the User

  *  Sterile 2.0ml microcentrifuge tubes                    * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g                 * Absolute ethanol

  Important notes

  • 1. Please add 15.75ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
  • 2. Please add 37.8ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.
  • 3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37℃ until the precipitate has fully dissolved.

  Experimental data:

  Analysis DNA

  • Absorbance analysis

  Get some DNA，diluted in a advisable factor with elution buffer. Survey the OD260，OD280 and OD320.

  Expressions：concentration（μg/ml）＝50×OD260×dilution fact

  Target：    2.1≥OD260-320/ OD280-320≥1.7

  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis

  0.8～1％ Agarose gel

  Example 1：Mouse heart and mouse muscle

  

  B: Bioer Kit

  O: Other company Kit

  Example 2：Mouse liver

  

  
  

   

  Manual download