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Tel: | 0571-87774513 | |
E-mail: | reagent@bioer.com.cn |
Fast Lysis Method
Cat# |
BSC59M1 |
Components |
100Tests |
BIOZOL Total RNA Extraction Reagent |
100 ml |
Handbook |
1 copy |
RNA Grind tube |
100 tubes |
BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.
Since the operation of BioFast Biozol is very easy, it can realize the extraction for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.
RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.
lPrepare RNA, dilute by 25mM Tris-HCl(pH7.5), RNase-free water or TE buffer in a right factor;
lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer;
lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:
Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)
lCalculate the purity of RNA as follows: Ratio=(Spec.readingA260)/( Spec.readingA280) Pictures of Experiment Picture 1:Total RNA extracted from animal tissues Picture 2 Total RNA extracted from rice leaves
(The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.)
Cat# |
BSC60S1 |
Components |
50Tests |
RNA Grind tube |
50 tubes |
Solution R2 |
30ml |
Wash Buffer |
18ml (add 54ml ethanol before use) |
Elution Buffer |
10ml |
Spin columns |
50 |
Handbook |
1copy |
Biofast-simplyP is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. Add sample to the grind tube with Solution R2 for homogenization and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.
The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-through put. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis、construction cDNA library etc.
Storage and transportation
Apparatus and materials to be prepared by the user
* Sterile 1.5ml microcentrifuge tubes * 10µl/200µl/1000µl tips
* Microcentrifuge capable of 14,000rpm * Absolute ethanol * Vortex mixer
Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.
lPrepared RNA will be dilute by 25mM Tris-HCl (pH7.5), RNase-free water or TE buffer in a right factor;
lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl(pH7.5), RNase-free water or TE buffer;
lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:
Final concentration = (Spec reading A260)×(Dilution factor)×(Conversion factor A260)
2. Notice: the range of Spec reading A260: 0.1≤OD260≤1.0
Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/( Spec.readingA280)
(The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.)
Picture 1:Animal tissues
Picture 2:Rice leaf
Cat# |
BSC21S1 |
Components |
50Tests |
SP Buffer |
45ml |
Lysis S Buffer |
5ml |
DA Buffer |
12.5ml |
Binding Buffer |
35ml |
Wash Buffer |
30ml(add 45ml absolute ethanol before use) |
Elution Buffer |
15ml |
Grind Tube |
50tubes |
Spin Column |
50tubes |
Handbook |
1copy |
The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.
At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.
Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.
All reagents, when stored properly, are stable for 18 months.
* Sterile 2.0ml microcentrifuge tubes * 10µl/100µl/1000µl tips
* Microcentrifuge capable of 14,000g * Absolute ethanol
Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.
Expressions:concentration(μg/ml)=50×OD260×dilution fact
Target: 2.0≥OD260-320/ OD280-320≥1.7
Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.
0.8~1% Agarose gel
Real-time Taqman PCR detect
Cat# |
BSC18S1 |
Components |
50Tests |
LP Buffer |
45 ml |
DA Buffer |
10 ml |
P Binding Buffer |
16ml |
G Binding Buffer |
25ml |
Wash Buffer |
25ml |
Elution Buffer |
10ml |
Spin Column |
50 |
Grind Tube |
50 |
Handbook |
1 copy |
The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from plant tissues, Designed for use with the lysis instrument from BIOER,plants are easily lysed within 40 s.The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this Kit.
The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.
* Sterile 1.5ml microcentrifuge tubes * 10µl/100µl/1000µl tips
* Microcentrifuge capable of 14,000g * Absolute ethanol * Vortex mixer
Get some DNA,diluted in a advisable factor with Elution Buffer. Survey the OD260,OD280 and OD320.
Expressions:concentration(μg/ml)=50×OD260×dilution fact
Target: 2.0≥OD260-320/ OD280-320≥1.7
Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.
0.8~1% Agarose gel
Example 1:Rice leaves
Example 2:Rice leaves
Cat# |
BSC20S1 |
Components |
50Tests |
FL Buffer |
30ml |
Binding Buffer |
35ml |
PW Buffer |
10.5ml |
Wash Buffer |
25.2ml |
Elution Buffer |
10ml |
PK Solution |
0.5ml |
WS buffer |
5ml |
Grind Tube |
50 tubes |
Spin Column |
50 tubes |
Handbook |
1 copy |
The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than1.5 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 30 μg genomic DNA can be acquired from up to 50 mg tissue by using this kit.
The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.
* Sterile 2.0ml microcentrifuge tubes * 10µl/100µl/1000µl tips
* Microcentrifuge capable of 14,000g * Absolute ethanol
Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.
Expressions:concentration(μg/ml)=50×OD260×dilution fact
Target: 2.1≥OD260-320/ OD280-320≥1.7
Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.
Example 1:Mouse heart and mouse muscle
B: Bioer Kit
O: Other company Kit
Example 2:Mouse liver
Manual download
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